GSM5983428: HCT116-RAD-mAC-auxin-12h-RAD21-ChIP-seq-Rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
RAD21
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
HCT116-RAD-mAC-auxin
cell line
HCT116-RAD-mAC
treatment
auxin-12h
method
RAD21-ChIP-seq
antibody
RAD21 (Abcam, catalog# ab922)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The fixed cells were lysed. The chromatin was sonicated to generate DNA fragments averaging 200-500 bp in length . We followed the protocol in the KAPA HyperPrep kit for the library preparation. The adaptors were ligated at the two ends of DNA fragments. The DNA fragments with adaptors will be amplified.