Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RAD21

Cell type

Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
HCT116-RAD-mAC-auxin
cell line
HCT116-RAD-mAC
treatment
auxin-12h
method
RAD21-ChIP-seq
antibody
RAD21 (Abcam, catalog# ab922)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The fixed cells were lysed. The chromatin was sonicated to generate DNA fragments averaging 200-500 bp in length . We followed the protocol in the KAPA HyperPrep kit for the library preparation. The adaptors were ligated at the two ends of DNA fragments. The DNA fragments with adaptors will be amplified.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
99661912
Reads aligned (%)
98.9
Duplicates removed (%)
42.4
Number of peaks
5005 (qval < 1E-05)

hg19

Number of total reads
99661912
Reads aligned (%)
98.1
Duplicates removed (%)
43.2
Number of peaks
4337 (qval < 1E-05)

Base call quality data from DBCLS SRA